Polymerase Chain Reaction (PCR) allows the user to amplify DNA sequences with known flanking regions. It is a mainstay method in molecular research and diagnostics.
Gene And Cell Technologies' PCR MasterMix (5x) provides all the components necessary to perform PCR, including Taq Polymerase, Buffers and dNTPs. Just provide template DNA and primers to direct the amplification to your gene of interest, and you will be on your way.
Our newly upgraded PCR MasterMix formulation is stable at ambient temperature during shipping. It should be stored in the fridge for up to 12 months, and frozen at -80C for the very long term. We have tested up to 18 months in the fridge, and up to 20 freeze-thaw cycles in the freezer. No deterioration of signal by qPCR was detected under either condition.
The PCR Master Mix is stable over a wide range of temperatures and ships at ambient temperature. This procedure has undergone extensive testing, and we found no adverse effects on PCR activity, in qPCR and regular reactions, even after shipping it across the country and back multiple times.