Your Cart(0)

PCR MasterMix (5x)

by Gene And Cell Technologies
Price $50.00

Polymerase Chain Reaction (PCR) allows the user to amplify DNA sequences with known flanking regions.  It is a mainstay method in molecular research and diagnostics.  Gene And Cell Technologies' PCR MasterMix (5x) provides all the components necessary to perform PCR, including Taq Polymerase, Buffers and dNTPs.  Just provide template DNA and primers to direct the amplification to your gene of interest, and you will be on your way.  

This PCR master mix is suitable for amplifications from plasmid, cDNA and genomic templates, by regular or qPCR. 

 

Specification

Sensitivity:  Better than 150 copies of our test plasmid in a 20 ul qPCR reaction (shown below under "Typical Results").  Baseline (zero template) must be flat.

 

Typical Results

Note:  For maximum flexibility, this PCR master mix does not contain any dye.  To use it in qPCR, please add your own dye or probe according to your experimental needs. 

 

Shipping & Storage

PCR MasterMix from Gene And Cell Technologies is stable at ambient temperature during shipping.  It is stable in the fridge for at least 12 months, and should be frozen at -80C for the very long term.  Stability has undergone extensive testing.  We found no adverse effects on PCR activity, in qPCR and regular reactions, even after shipping it across the country and back multiple times.  

 

Pipetting Plan:  20 ul PCR reaction

Water.....................................14.8 ul 

PCR MasterMix (5x).............. 4.0 ul

Primer F (10 uM).................. 0.4 ul

Primer R (10 uM).................. 0.4 ul

Template................................0.4 ul


Some of the water volume may be replaced with other reagents as needed.  This could be an increased amount of template (assuming it is clean and free of PCR inhibitors), or a dye or probe for qPCR.  The entire reaction can be scaled to different volumes, e.g. 50 ul, by multiplying all the volumes accordingly.  In line with industry standards, we specify the product amount in reference to 50 ul reaction size. 

 

 

We Accept...

We Accept Visa We Accept Discover We Accept American Express We Accept Mastercard We Accept PayPal We Accept Google Checkout