DNA Ladders are used as standards for estimating the size of DNA fragments in agarose gel electrophoresis. While DNA size estimation is generally going to be robust, the following tips can help you get the very highest accuracy:
- Load 10 ul per lane for optimal, publication-grade images. In routine use, it is possible to load substantially less and still get good results.
- The Ladder is provided in 5% glycerol. Use the same for your samples for optimal sizing.
- The amount of sample loaded (in ng) should be with in an order of magnitude of the total ladder amount loaded.