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Did you know?

Adjusting your pH by adding acid or base creates salt! That affects the salt concentration of your solution in ways that can be difficult to understand, and that can have unexpected consequences for your experiment.

The equation of Henderson & Hasselbalch offers a way to create buffered solutions by weighing in the exact amounts of acid and base that you need to achieve your desired pH. It's the best way to create buffered solutions with a defined salt concentration. Unfortunately, the equation itself is a bit unwieldy. That's why we have created this user-friendly online tool to do the heavy calculation for you.

Our calculator knows the molecular weights, pKa and possible hydration states of the most common molecular biology buffers. Just select yours from the list and let our website do the calculations for you. (Or, if your buffer is not in the system yet, select "custom".  This allows you to enter your own parameters).

Instructions:

(1) Select your buffer system, acid and base.

(2) The system automatically populates the relevant fields, like Mw and pKa.

(3) Enter your requirements for pH and concentration.

 => Read off exactly how much acid and base you need to weigh.

 

buffer system:
acid:
acid Mw:
g/mol
base:
base Mw:
g/mol
Enter your requirements: pH:
Concentration:
M
Volume:
L
pKa:

 



 

Results:
You need Acid mass:
g
Base mass:
g

 

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