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SparkleCell

SparkleCell is our family of bioreactors for high yield production of proteins and plasmids in E. coli.  The starter model is the 2-liter SparkleCell EasyGrow.  Other models are currently built to order based on specific customer needs.  Some of the possibilities are shown below: 

SparkleCell Bioreactor E. coli yield models comparison bubble column

 

Benefits of SparkleCell:

  • High oxygenation level translates into healthy cells producing lots of product.
  • Detailed protocols and assistance with plasmid design available (upon purchase). 
  • Seamlessly scale up to larger models.  The physics and protocols don't change. 
  • No more expensive than an equivalent large volume shaker. 
  • Fun to use, looks great on your lab bench or in your process room.

 

Unsure about SparkleCell?

Today all our commercial E. coli production runs on SparkleCell, and we cannot imagine touching a large shaker flask ever again.  However, it is understandable that those used to shaker-flask culture have many questions.  There are two ways to evaluate SparkleCell for your project with minimal commitment:

(1) Hire us to make your protein in SparkleCell as a Service.  We can send you cell pellets (they're huge!) and optionally purify, characterize etc.  Let us know what you need.  We're flexible. 

(2) Purchase a 2L SparkleCell EasyGrow.  EasyGrow is used for testing and method development, and is generally predictive of the larger models.  It costs less than a large capacity incubated shaker.

 

Caveats

 * Protein yield requires optimal gene design and a protein that lends itself to E. coli expression.  SparkleCell cannot make constructs work that don't work in shaker flasks.  But it can usually improve the volumetric yield and ease of production dramatically, for constructs that work well in shaker flasks.  

** Plasmid yield assumes a high copy number origin of replication, such as pUC, and absence of difficult features, such as inversions, repeats, extreme G/C content.  The yield of pUC plasmids is often proportional to their size.  Again, SparkleCell will often improve the yield, and certainly the convenience of production seen in shaker flasks.  But it cannot make constructs work that don't work reasonably well in flasks. 

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