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Purification of Thermostable DNA Polymerases

Thermostable polymerases are of course key enablers of molecular biology, due to their usefulness in polymerase chain reaction (PCR).  Today I am going to show you the process by which these important enzymes are isolated.  You will learn how to make your own Taq polymerase using the open source expression vector pOpenTaq, as well as understand the purity grades of the commercial polymerases offered by Gene And Cell Technologies.  The principles outlined in this blog post should apply to any thermostable nucleic acid polymerase.   Taq polymerase can be expressed form pOpenTaq, an open-source taq polymerase expression plasmid.  pOpenTaq should..  - Read More